Microbiology QUIZ Part V – CULTURE MEDIA & METHOD| GPAT, Drug Inspector, DCO, Pharmacist, EXIT, NIPER

/26

Created on August 19, 2022 By

SS Jakhar

Microbiology

Microbiology QUIZ Part V- CULTURE MEDIA & METHODS

1 / 26

Yeast extract is prepared from

Casein

lean meat

heart muscle

Saccharomyces

2 / 26

Which of the following is not true about peptone.

It is hygroscopic

Obtain from lean meat, heart muscle, casein

Mainly supply nitrogenous material

None of the above.

3 / 26

Which of the following is not a storage media.

Egg saline medium

Dorsets egg medium

Blood agar slants

None

4 / 26

In chocolate agar media the concentration of blood is

5 -10%

1 to 2 %

20 to 30%

0.5 to 1%

5 / 26

A culture medium the exact composition of which is not known was called as

Synthetic media

Complex media

Defined Media

Natural media

6 / 26

Which of the following is a characteristic of beef extract?

Product resulting from the digestion of proteinaceous materials

Aqueous extract of lean beef tissue

Aqueous extract of yeast cells

Complex carbohydrate obtained from certain marine algae

7 / 26

Which of the following is a rich source of B vitamins?

Peptone

Yeast extract

Beef extract

Agar

8 / 26

Agar media can be enriched with blood for the identification of?

Bacteria which can grow in anaerobic conditions

Bacteria which can cause hemolysis

Viruses from other microorganisms

Bacteria which can grow in higher hydrostatic pressures

9 / 26

Culture medium used for growth of fungi is

Nutrient agar media

Sabouraud’s medium

Nutrient broth

Minimal agar medium

10 / 26

In the medium other than nutrients, if any substance is used in excess, that medium is

Indicator medium

Special medium

Enriched medium

Sugar medium

In the medium other than nutrients, if any substance is used in excess, that medium is called as Enriched medium

11 / 26

Which of the following is not true about agar?

Source of nutrient

Long chain Polysaccharide

Melts at 95 to 98 degree Centigrade

Solidies at approx. 40 degree Centigrade

12 / 26

Which of the following is the example of Anaerobic medium

Robertson cooked-meat medium

Nutrient agar

Nutrient broth

Mac-Conkey’s agar

13 / 26

For preparation of semi-solid media agar used in concentration between

1 to 2 %

1.5 to 2.5 %

0.2 to 0.5%

0.05 to 0.009%

14 / 26

What is the concentration of agar in solid media

3 to 4 %

1.5 to 2 %

10 t0 15 %

0.5 to 1%

15 / 26

Example of indicator medium is

Nutrient Agar Medium

Mac-Conkey’s agar Medium

Wilson and Blair Medium

Czapeck-dox medium

16 / 26

Blood agar media is used for the growth of?

Fungi

Fastidious bacteria

Halophiles

Red algae only

17 / 26

The most commonly used solidifying agent Agar is obtained from

Brown algae

Red algae

Green alga

Blue-green algae

18 / 26

Peptone water and nutrient broth are

Enriched media

Enrichment media

Basal media

Differential medium

19 / 26

Blood agar medium is

Enrichment medium

Enriched medium

Selective medium

Differential medium

20 / 26

The solidifying agent used in microbiology laboratories to solidify medium

Cellulose

Peptone

Thioglycollate

Agar

21 / 26

Which of the following bacteria produce saccharolytic reaction in cooked meat broth (CMB)?

Costridium perfrngens

Costridium tetani

Pseudomonas aeruginosa

None of the above

22 / 26

Anaerobic jar is commonly used to grow:

Costridium tetani

Klebsiella sp.

Pseudomonas aeruginosa

None of the above

23 / 26

Which enrichment medium is preferred to grow Vibrio cholerae?

Tetrathionate broth

Selenite F broth

All of the above

24 / 26

When a liquid medium contains a substance that inhibits all unwanted bacteria but stimulates the growth of particular bacteria, it is known as:

Selective medium

Enriched medium

Enrichment medium

None

25 / 26

When a solid medium contains a substance that inhibits all unwanted bacteria but facilitates the isolation of particular bacteria, it is known as:

Selective medium

Enriched medium

Indicator medium

None of the above

26 / 26

All of the following are examples of enriched media except:

Blood agar

Chocolate agar

Loeffler’s serum slope

Bile salt agar

Your score is

The average score is 30%

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Category: Microbiology

175

Created on December 27, 2021 By

SS Jakhar

ANTISEPTIC AND DISINFACTAN MCQ Quiz-Pharmapedia

Antiseptic and Disinfectant-Microbiology MCQ Quiz | GPAT Pharmacist DI NIPER

1 / 20

Temperature & time used in holder method of Pasteurization is

63°C for 30 minutes

63°C for 50 minutes

72°C for 20 seconds

72°C for 20 minutes

2 / 20

Temperature & time period used in hot air oven is

160°C for 2 hrs

140°C for 2 hrs

160°C for 1 hr

None

3 / 20

Chemical disinfectants which can be safely applied to living tissue to prevent infection are known as

Formaldehyde

Antiseptics

Ethylene oxide

None of the above

4 / 20

The process by which on article medium or surface is made free of Microorganism/MO is known as

Sterilization

Disinfection

Asepsis

Pasteurization

5 / 20

Disposable syringes are sterilized by

Autoclave

Gamma radiations

Filtration

Incineration

6 / 20

Antiseptic action of alcohol mainly due to

denaturing the protein

Oxidation of protein

Interfere with nucleic acid synthesis

None

7 / 20

Which of the following is a non-ionization radiation used in disinfectant/sterilization

Gamma rays

X-rays

UV radiation

Cosmic rays

8 / 20

In Tyndallisation process user for sterilization of egg, serum media involves

Heat at 100°C for 20 minutes on three successive days

Heat at 100°C for 20 minutes on two successive days

80 - 85°C for 30 minutes for 3 consecutive days

none

9 / 20

Flash method of Pasteurization

63°C for 30 minutes

72°C for 20 sec followed by cooling quickly to 13°C or lower

80 - 85°C for 30 minutes

At 100°C

10 / 20

Biological indicator used in moist heat sterilization method to check effectiveness of a given sterilization method

B. subtilis

Bacillus streareothermophilus

Bacillus pumilus

Pseudomonas dimunuta

11 / 20

Cold sterilization is also know as

Gamma radiation

Autoclave

Hot air oven

None

12 / 20

Physical Integrity test for disc membrane filter is checked by

Bubble point test

Clarity test

DOP test

both a & c

13 / 20

In HEPA filter, HEPA stands for ...

High efficiency paper air

High effective particulate air

High efficiency particulate air

High efferent particulate aerosol

14 / 20

Porosity of membrane filter medium in the process of sterilization by filtration

0.45 μm

0.22 μm

1 μm

2 μm

15 / 20

In dry heat Sterilization holding temp & time

160°C & 2 hrs

170°C & 1 hr

180°C & 30 minutes

All of these

16 / 20

. Sterilization of Non-aqueous thermostable can be carry out using

Autoclave

Dry heat Sterilization

Ethylene oxide gas

Radiation

17 / 20

Holding temperature & time in the moist heat sterilization (Autoclave)

160°C for 2 hrs

650°C for 1 minutes

121°C for 15 minutes

63°C for 30 minutes

18 / 20

To sterile aqueous & surgical materials which one of the following method would be preferred

moist heat sterilization

Radiation method

Dry heat sterilization

Membrane filtration method

19 / 20

Culture media use to carry out sterility test of parenteral & ophthalmic preparation

Fluid thioglycolate medium

Soyabean case in digest medium

Alternative thioglycolate medium

all of these

20 / 20

Sterility test for sterile preparation are carried out by

Membrane filter method

Direct innoculation methodethod

Pyrogen test

Rabbit test

both a & b

Your score is

The average score is 45%

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Created on August 14, 2022 By

SS Jakhar

Microbiology

Created on December 27, 2021 By

SS Jakhar

Antiseptic & Disinfectant MCQ Quiz part II- GPAT Pharmacist NIPER DI

1 / 22

Which of the following methods is useful for sterilization of antisera?

Filtration

Hot air oven

Tyndallisation

Autoclaving

2 / 22

Browne’s tube is used as indicator for efficacy of:

Ultraviolet rays

Heat sterilization

Filtration

Chemical sterilization

3 / 22

The efficiency of moist-heat sterilization is tested with

Vegetative cells of Bacillus stearothermophilus

pores of Bacillus stearothermophilus

Spores of aerobic bacteria

Clostridium botulinum

4 / 22

All of the following disinfectant contains heavy metal EXCEPT

Mercurochrome

Chlorine

Copper sulfate

Merthiolate

Silver nitrate

5 / 22

All of the following are sporicidal except

ethylene oxide

stabilized hydrogen peroxide

formaldehyde

glutaraldehyde

alcohol

6 / 22

Which of the following method is best to sterilize heat labile solutions?

Autoclave

Membrane filtration

Pasteurization

Hot Air Oven

7 / 22

Which of the following was the first widely used antiseptic and disinfectant?

Iodine

Alcoholes

Phenol

Chlorine

8 / 22

Identify the term that describes a disinfectant that can kill bacteria

Bactericidal

Bacteriostatic

Pathogenic

Bacteriosis

9 / 22

Identify the term that describes an environment completely free of microorganisms:

Antibiotic

Asepsis

Antisepsis

None

10 / 22

Identify the correct definition of an antiseptic:

Chemicals that kill or prevent infection and damage living tissues

Chemicals that kill or prevent infection without damaging living tissues

Chemicals that can only kill or prevent infection on non-living tissues

Chemicals that can only kill or prevent infection on animal housing

11 / 22

Isopropyl alcohol show maximum antiseptic activity

20-30 %

60-70 %

35 %

99.9 %

12 / 22

Cidex contains

Chlorhexidine and Cetrimide

Glutaraldehyde

Cresol

Chloroxylenol

13 / 22

Efficacy testing of Disinfectant is carries out by

Carrier test

Suspension tests

Capacity test

Field Test

All of these

14 / 22

Test organism used in the phenol coefficient road, test organism is

B. subtilis

Salmonell typhi

B pumilus

Pseudomonas diminuta

15 / 22

Which of the following is a/an intermediate level disinfectant?

Glutaraldehyde

peracitic acid

Hydrogen peroxide

Alcohols

16 / 22

R-W coefficient test is used to evaluate

Antibiotic activity

Sterility of packaging material

Nature of organism in bacterial infection

Bactericidal activity

17 / 22

Which of the following is used commercially for sterilization of disposable plastic items

Ethylene oxide

Autoclave

Glutaraldehyde

Ethyl alcohol

18 / 22

Glutaraldehyde is used for sterilization of

Cystoscopes

Endoscopes

Broncho scopes

All of these

19 / 22

Gamma radiation can be used for sterilization of the followings

Plastic syringes

Catheter

Swabs

all of these

20 / 22

Which bacteria spores are used in hot air oven sterilization

Stearothemophilus

Bacillus subtilis subsp niger

Pseudomonas aeroginosa

Catheters

21 / 22

Steam sterilization at 100 for 20 minutes on 3 successive days is known as

Tyndallisation

Inspissation

Pasteurization

Vaccine bath

22 / 22

Bacteria that can survive in holder method of Pasteurization

Bordetella pertussis

Coxiella burnetii

Salmonella typhi

Myobacterium bovis

Your score is

The average score is 39%

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/12

Created on August 18, 2022 By

SS Jakhar

Microbiology

Microbiology QUIZ Part IV-STERILISATION AND DISINFECTION

1 / 12

The process by which an article, surface or medium is made free of all microorganisms either in the vegetative spore form is known as:

A) Sterilisation

B) Disinfection

C) Asepsis

D) None of the above

2 / 12

Chemical disinfectants which can be safely applied to living tissues to prevent infection are known as:

A) Antiseptics

B) Formaldehyde

C) Ethylene oxide

D) None of the above

3 / 12

Temperature and time period used in hot air oven is:

A) 160℃ for two hours

B) 140℃ for two hours

C) 160℃ for one hours

D) None of the above

4 / 12

Temperature and time period used in holder method of Pasteurization is

A) 63℃ for 30 minutes

B) 63℃ for 50 minutes

C) 72℃ for 20 seconds

D) 72℃ for 40 seconds

5 / 12

Which of the following bacteria can survive in holder method of Pasteurization?

A) Bordetella pertussis

B) Coxiella burnetii

C) Salmonella typhi

D) Mycobacterium bovis

6 / 12

Stream sterilization at 100 for 20 minutes on three successive days is known as

A) Tyndallisation

B) Inspissation

C) Pasteurisation

D) Vaccine bath

7 / 12

Which of the following sterilization conditions are used in autoclave?

A) 121℃, 15 pounds pressure/sq inch, 15 minutes

B) 160℃, 20 pounds pressure/sq inch, 20 minutes

C) 140℃, 10 pounds pressure/sq inch, 15 minutes

D) None of the above

8 / 12

Which bacterial spores are used as sterilization control in autoclave?

A) Clostridium perfringens

B) Bacillus cereus

C) Bacillus stearothermophilus

D) Pseudomonas aeruginosa

9 / 12

Gamma radiations can be used for sterilization of:

A) Plastic syringes

B) Catheters

C) Swabs

D) All of the above

10 / 12

Glutaraldehyde is used for sterilization of

A) Cystoscopes

B) Endoscopes

C) Bronchoscopes

D) All of the above

11 / 12

The most widely used disinfectant for human immunodeficiency virus (HIV) infected material is

A) Hypochlorite solution

B) Lysol

C) Formaldehyde

D) Mercuric chloride

12 / 12

Which of the following is used commercially for sterilization of disposable plastic items?

A) Ethylene oxide

B) Autoclave

C) Glutaraldehyde

D) Ethyl alcohol

Your score is

The average score is 56%

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133

Created on August 25, 2022 By

SS Jakhar

Microbiology

Microbiology QUIZ Part X– IMMUNITY & VACCINE

NameEmailPhone Number

1 / 14

Which of the following immunity is called the first line of defence?

a) Innate Immunity

b) Active immunity

c) Passive immunity

d) Acquired immunity

2 / 14

Cells Involved In Innate Immunity are

a) Phagocytes

b) Natural Killer Cells

c) Macrophages

d) All of the above

3 / 14

Skin, body hair, cilia, eyelashes, the respiratory tract and the gastrointestinal tract are examples of

a) Physical barrier

b) Cellular barriers

c) Cytokine barriers

d) Physiological barriers

4 / 14

Which of the following cells is involved in humoral immunity?

a) T-cells

b) B-cells

c) B-cells

d) Both T and B cells

5 / 14

Which of the following conveys the longest-lasting immunity to an infectious agent?

a) Active immunity

b) Passive immunity

c) Both (a) and (b)

d) None of the above

6 / 14

Which of the following cells is involved in cell-mediated immunity?

a) T-cells

b) B-cells

c) Mast cells

d) Both T and B cells

7 / 14

Which of the following statements is true about Passive Immunity?

a) This immunity causes reactions

b) This immunity develops immediately

c) This immunity lasts only for a few weeks or months

d) All of the above.

8 / 14

B-cells and T-cells are two types of cells involved in

a) Innate Immunity

b) Active immunity

c) Passive immunity

d) Acquired immunity

9 / 14

Which of the following immunity is present from our birth?

a) Innate Immunity

b) Active immunity

c) Passive immunity

d) Acquired immunity

10 / 14

All of the following are killed vaccines except

a) Salk vaccine

b) Hepatitis B vaccine

c) Non-neural vaccines for rabies

d) MMR

11 / 14

All of the following are live vaccines except:

a) BCG

b) Sabin vaccine

c) MMR

d) TAB vaccine

12 / 14

Artificial passive immunity may be induced by

a) Antiserum injection

b) Vaccination

c) Maternal antibodies

d) All of the above

13 / 14

Vaccination induces:

a) Active natural immunity

b) Passive natural immunity

c) Active artificial immunity

d) Passive artificial immunity

14 / 14

Clinical or subclinical infections lead to

a) Active natural immunity

b) Passive natural immunity

c) Active artificial immunity

d) Passive artificial immunity

Your score is

The average score is 37%

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Yeast extract is prepared from

Which of the following is not true about peptone.

Which of the following is not a storage media.

In chocolate agar media the concentration of blood is

A culture medium the exact composition of which is not known was called as

Which of the following is a characteristic of beef extract?

Which of the following is a rich source of B vitamins?

Agar media can be enriched with blood for the identification of?

Culture medium used for growth of fungi is

In the medium other than nutrients, if any substance is used in excess, that medium is

Which of the following is not true about agar?

Which of the following is the example of Anaerobic medium

For preparation of semi-solid media agar used in concentration between

What is the concentration of agar in solid media

Example of indicator medium is

Blood agar media is used for the growth of?

The most commonly used solidifying agent Agar is obtained from

Peptone water and nutrient broth are

Blood agar medium is

The solidifying agent used in microbiology laboratories to solidify medium

Which of the following bacteria produce saccharolytic reaction in cooked meat broth (CMB)?

Anaerobic jar is commonly used to grow:

Which enrichment medium is preferred to grow Vibrio cholerae?

When a liquid medium contains a substance that inhibits all unwanted bacteria but stimulates the growth of particular bacteria, it is known as:

When a solid medium contains a substance that inhibits all unwanted bacteria but facilitates the isolation of particular bacteria, it is known as:

All of the following are examples of enriched media except:

Category: Microbiology

In dry heat Sterilization holding temp & time

Which is the first human disease proved to be of viral origin?

Who introduced vaccination method for prevention of small pox?

Who proposed ‘side chain theory’ of antibody production?

Who discovered Mycobacterium tuberculosis?

Who introduced methods of obtaining bacteria in pure cultures using solid media?

The term vaccine was coined by:

Who introduced the techniques of sterilization?

Who is known as the Father of Microbiology?

Who is known as the ‘Father of Chemotherapy’?

The efficiency of moist-heat sterilization is tested with

The process by which an article, surface or medium is made free of all microorganisms either in the vegetative spore form is known as:

Chemical disinfectants which can be safely applied to living tissues to prevent infection are known as:

Temperature and time period used in hot air oven is:

Temperature and time period used in holder method of Pasteurization is

Which of the following bacteria can survive in holder method of Pasteurization?

Stream sterilization at 100 for 20 minutes on three successive days is known as

Which of the following sterilization conditions are used in autoclave?

Which bacterial spores are used as sterilization control in autoclave?

Gamma radiations can be used for sterilization of:

Glutaraldehyde is used for sterilization of

The most widely used disinfectant for human immunodeficiency virus (HIV) infected material is

Which of the following is used commercially for sterilization of disposable plastic items?

Which of the following immunity is called the first line of defence?

Cells Involved In Innate Immunity are

Skin, body hair, cilia, eyelashes, the respiratory tract and the gastrointestinal tract are examples of

Which of the following cells is involved in humoral immunity?

Which of the following conveys the longest-lasting immunity to an infectious agent?

Which of the following cells is involved in cell-mediated immunity?

Which of the following statements is true about Passive Immunity?

B-cells and T-cells are two types of cells involved in

Which of the following immunity is present from our birth?

All of the following are killed vaccines except

All of the following are live vaccines except:

Artificial passive immunity may be induced by

Vaccination induces:

Clinical or subclinical infections lead to

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